[Plain Grid] [Film Coated Grid] [Holey Film Coated Grid] [Lacey Film Coated Grid] [Quantifoil Film Coated Grid] [Grid Storage Box]

1. Plain Grids:

 

The diameter of grids is standard, as 3.05mm. The Thickness is variable based on the grid materials. The thickness range is from 10um to 25um. The mesh of the grid is defined by the number of hole within 1 inch. For example, the 200 mesh grid has 20 holes along diameter direction,  400 mesh grid has 40 holes in diameter.

 

Since the diameter of grid is a standard length, 3.05mm,  each hole size is less than ~200um in 100 mesh grid,  ~97um in 200 mesh grid, ~63um in 300 mesh grid and ~42um in 400 mesh grid.

 

Please be noticed that our grids have very smooth hole edge. The smooth edge grids could provide a very flat film coated on them, which is critical for high resolution imaging.

 

600 mesh 500 mesh 400 mesh 300 mesh 200 mesh 100 mesh 50 mesh Single hole Slot hole

Copper

(Cu-600)

Cu/Rh

(Cu/Rh-600)

Nickel

 (Ni-600)

Copper

(Cu-500)

Nickel

 (Ni-500)

Copper

(Cu-400)

Cu/Rh

(Cu/Rh-400)

Molybdenum

 (Mo-400)

Nickel

 (Ni-400)

Titanium

(Ti-400)

Copper

(Cu-300)

Cu/Rh

(Cu/Rh-300)

Molybdenum

 (Mo-300)

Nickel

 (Ni-300)

Titanium

(Ti-300)

Steel

(Fe-300)

Copper (Cu-200)

Cu/Rh (Cu/Rh-200)

Molybdenum (Mo-200)

Nickel (Ni-200)

Titanium

(Ti-200)

Gold (Au-200)

Tungsten (W-200)

Steel (Fe-200)

Nylon (Ny-200)

Copper (Cu-100)

Cu/Rh (Cu/Rh-100)

Molybdenum (Mo-100)

Nickel (Ni-100)

Titanium

(Ti-100)

Gold (Au-100)

Steel (Fe-100)

Copper (Cu-50)

Molybdenum (Mo-50)

Nickel (Ni-50)

Titanium

(Ti-50)

Gold (Au-50)

Steel (Fe-50)

Copper

(Cu-s)

Molybdenum

(Mo-s)

Copper

(Cu-50/200)

(Cu-75-300)

(Cu-100/400)

(click each item to link to the details information)

 

600 mesh 500 mesh 400 mesh 300 mesh 200 mesh 100 mesh 50 mesh single hole slot hole

 

Purchase Order Information

 

mesh prod # materials hole size bar size packed price  
600 mesh Cu-600 copper ~18Ám ~23Ám 50/vial $29.99
600 mesh Cu/Rh-600 copper/rhodium ~18Ám ~23Ám 50 vial $29.99
600 mesh Ni-600 nickel ~18Ám ~23Ám 50/vial $29.99
Purchase Order Information
500 mesh Cu-500 copper ~28Ám ~24Ám 50/vial $29.99
500 mesh Ni-500 nickel ~28Ám ~24Ám 50/vial $29.99
Purchase Order Information
400 mesh Cu-400 copper ~42Ám ~24Ám 50/vial $19.99
400 mesh Cu/Rh-400 copper/rhodium ~42Ám ~24Ám 50 vial $19.99
400 mesh Mo-400 Molybdenum ~42Ám ~24Ám 50/vial $199.99
400 mesh Ni-400 nickel ~42Ám ~24Ám 50/vial $19.99
400 mesh Ti-400 Titanium ~42Ám ~24Ám 25/vial $79.99
400 mesh Fe-400 Stainless Steel ~42Ám ~24Ám 25/vial $79.99
400 mesh Au-400 Gold ~42Ám ~24Ám 25/vial $39.99
Purchase Order Information
300 mesh Cu-300 copper ~63Ám ~25Ám 50/vial $19.99
300 mesh Cu/Rh-300 copper/rhodium ~63Ám ~25Ám 50 vial $19.99
300 mesh Mo-300 Molybdenum ~63Ám ~25Ám 50/vial $199.99
300 mesh Ni-300 nickel ~63Ám ~25Ám 50/vial $19.99
300 mesh Ti-300 Titanium ~63Ám ~25Ám 25/vial $79.99
300 mesh Fe-300 Stainless Steel ~63Ám ~25Ám 25/vial $79.99
300 mesh Au-300 Gold ~63Ám ~25Ám 25/vial $39.99
Purchase Order Information
200 mesh Cu-200 copper ~97Ám ~26Ám 50/vial $19.99
200 mesh Cu/Rh-200 copper/rhodium ~97Ám ~26Ám 50 vial $19.99
200 mesh Mo-200 Molybdenum ~97Ám ~26Ám 50/vial $199.99
200 mesh Ni-200 nickel ~97Ám ~26Ám 50/vial $19.99
200 mesh Ti-200 Titanium ~97Ám ~26Ám 25/vial $79.99
200 mesh Fe-200 Stainless Steel ~97Ám ~26Ám 25/vial $79.99
200 mesh Au-200 Gold ~97Ám ~26Ám 25/vial $39.99
200 mesh W-200 Tungsten ~97Ám ~26Ám 25/vial $249.99
200 mesh Ny-200 Nylon ~97Ám ~26Ám 25/vial $89.99
Purchase Order Information
100 mesh Cu-100 copper ~200Ám ~50Ám 50/vial $19.99
100 mesh Cu/Rh-100 copper/rhodium ~200Ám ~50Ám 50 vial $19.99
100 mesh Mo-100 Molybdenum ~200Ám ~50Ám 50/vial $199.99
100 mesh Ni-100 nickel ~200Ám ~50Ám 50/vial $19.99
100 mesh Ti-100 Titanium ~200Ám ~50Ám 25/vial $79.99
100 mesh Fe-100 Stainless Steel ~200Ám ~50Ám 25/vial $79.99
100 mesh Au-100 Gold ~200Ám ~50Ám 25/vial $39.99
Purchase Order Information
50 mesh Cu-50 copper ~425Ám ~83Ám 50/vial $19.99
50 mesh Cu/Rh-50 copper/rhodium ~425Ám ~83Ám 50 vial $19.99
50 mesh Mo-50 Molybdenum ~425Ám ~83Ám 50/vial $199.99
50 mesh Ni-50 nickel ~425Ám ~83Ám 50/vial $19.99
50 mesh Ti-50 Titanium ~425Ám ~83Ám 25/vial $79.99
50 mesh Fe-50 Stainless Steel ~425Ám ~83Ám 25/vial $79.99
50 mesh Au-50 Gold ~425Ám ~83Ám 25/vial $39.99
Purchase Order Information
single hole Cu-s copper ~1500Ám - 50/vial $19.99
single hole Mo-s Molybdenum ~1500Ám - 50/vial $199.99
Purchase Order Information
50/200 mesh Cu-50200 Cupper ~425X87Ám ~40Ám 50/vial $39.99
75/300 mesh Cu-75300 Cupper ~288X61Ám ~20Ám 50/vial $39.99
100/400 mesh Cu-100400 Cupper ~204X40Ám ~23Ám 50/vial $39.99

 

 

materials mesh prod # hole size bar size packed price  
copper 600 mesh Cu-600 ~18Ám ~23Ám 50/vial $29.99
copper 500 mesh Cu-500 ~28Ám ~24Ám 50/vial $29.99
copper 400 mesh Cu-400 ~42Ám ~24Ám 50/vial $19.99
copper 300 mesh Cu-300 ~63Ám ~25Ám 50/vial $19.99
copper 200 mesh Cu-200 ~97Ám ~26Ám 50/vial $19.99
copper 100 mesh Cu-100 ~200Ám ~50Ám 50/vial $19.99
copper 50 mesh Cu-100 ~425Ám ~83Ám 50/vial $19.99
copper single hole Cu-s ~1500Ám - 50/vial $19.99
50/200 mesh Cu-50200 Cupper ~425X87Ám ~40Ám 50/vial $39.99
75/300 mesh Cu-75300 Cupper ~288X61Ám ~20Ám 50/vial $39.99
100/400 mesh Cu-100400 Cupper ~204X40Ám ~23Ám 50/vial $39.99
Purchase Order Information
copper/rhodium 600 mesh Cu/Rh-600 ~18Ám ~23Ám 50 vial $29.99
copper/rhodium 400 mesh Cu/Rh-400 ~42Ám ~24Ám 50 vial $19.99
copper/rhodium 300 mesh Cu/Rh-300 ~63Ám ~25Ám 50 vial $19.99
copper/rhodium 200 mesh Cu/Rh-200 ~97Ám ~26Ám 50 vial $19.99
copper/rhodium 100 mesh Cu/Rh-100 ~200Ám ~50Ám 50 vial $19.99
copper/rhodium 50 mesh Cu/Rh-50 ~425Ám ~83Ám 50 vial $19.99
Purchase Order Information
Molybdenum 400 mesh Mo-400 ~42Ám ~24Ám 50/vial $199.99
Molybdenum 300 mesh Mo-300 ~63Ám ~25Ám 50/vial $199.99
Molybdenum 200 mesh Mo-200 ~97Ám ~26Ám 50/vial $199.99
Molybdenum 100 mesh Mo-100 ~200Ám ~50Ám 50/vial $199.99
Molybdenum 50 mesh Mo-50 ~425Ám ~83Ám 50/vial $199.99
Molybdenum single hole Mo-s ~1500Ám - 50/vial $199.99
Purchase Order Information
nickel 600 mesh Ni-600 ~18Ám ~23Ám 50/vial $29.99
nickel 500 mesh Ni-500 ~28Ám ~24Ám 50/vial $29.99
nickel 400 mesh Ni-400 ~42Ám ~24Ám 50/vial $19.99
nickel 300 mesh Ni-300 ~63Ám ~25Ám 50/vial $19.99
nickel 200 mesh Ni-200 ~97Ám ~26Ám 50/vial $19.99
nickel 100 mesh Ni-100 ~200Ám ~50Ám 50/vial $19.99
nickel 50 mesh Ni-50 ~425Ám ~83Ám 50/vial $19.99
Purchase Order Information
Titanium 400 mesh Ti-400 ~42Ám ~24Ám 25/vial $79.99
Titanium 300 mesh Ti-300 ~63Ám ~25Ám 25/vial $79.99
Titanium 200 mesh Ti-200 ~97Ám ~26Ám 25/vial $79.99
Titanium 100 mesh Ti-100 ~200Ám ~50Ám 25/vial $79.99
Titanium 50 mesh Ti-50 ~425Ám ~83Ám 25/vial $79.99
Purchase Order Information
Stainless Steel 400 mesh Fe-400 ~42Ám ~24Ám 25/vial $79.99
Stainless Steel 300 mesh Fe-300 ~63Ám ~25Ám 25/vial $79.99
Stainless Steel 200 mesh Fe-200 ~97Ám ~26Ám 25/vial $79.99
Stainless Steel 100 mesh Fe-100 ~200Ám ~50Ám 25/vial $79.99
Stainless Steel 50 mesh Fe-50 ~425Ám ~83Ám 25/vial $79.99
Purchase Order Information
Gold 400 mesh Au-400 ~42Ám ~24Ám 25/vial $39.99
Gold 300 mesh Au-300 ~63Ám ~25Ám 25/vial $39.99
Gold 200 mesh Au-200 ~97Ám ~26Ám 25/vial $39.99
Gold 100 mesh Au-100 ~200Ám ~50Ám 25/vial $39.99
Gold 50 mesh Au-50 ~425Ám ~83Ám 25/vial $39.99
Purchase Order Information
Tungsten 200 mesh W-200 ~97Ám ~26Ám 25/vial $249.99
Purchase Order Information
Nylon 200 mesh Ny-200 ~97Ám ~26Ám 25/vial $79.99

 

 

2. Continue Film Coated Grids:

 

Most samples for transmission electron microscopy must be "supported" on some kind of a thin electron transparent film, to hold the specimen in place while in the objective lens of the TEM. Only samples that are "self- supporting" do not need some additional support film. Should an electron microscope user use a support film at all if one is not absolutely needed? Probably not, because even the very best quality of support films do result in more material being placed in the beam's path, resulting even if ever so slightly, the contrast of the final image. But for many users, they don't have the luxury of having to make that decision: More often than not, even what appear to be self-supporting samples, still do need the assistance of a support film to cut down on sample drift.

Mo-300CN, Molybdenum grid coated with thin carbon film.

 

The selection of the "right" support film has never been an easy exercise, even the most experienced of TEM experts, and the only way to really determine which support film is the best for a particular application it to just "try it!" Therefore, we would like to contribute in a useful way to the needed information base to help make the optimum selections, but the giving of such "guidance" itself is an imprecise science.

We have therefore broken down the different kinds of support films and have tried to present the "trade offs" between one support film system vs. another. We can offer no guarantees, since the range of samples studied is so wide and the environmental range so broad (from cryo temperatures to temperatures exceeding 1000░C) to which the grids and their coatings might be subjected. But the best decisions are usually made by just trying several possibilities and if one works better than the other, the choice for future samples becomes obvious.


Following table will help you determine what support continue film to use for your particular transmission electron microscopy project. Once you have decided which substrate best suits your needs, you can go on on this page for a listing of available support films and pricing.

 

Application

1

Thin carbon film

2

Thick carbon film

3

Formvar film

4

Carbon/Formvar film

Protein, single particle, negative stained

good

good

unsuitable

good

Protein, single particle, cryo

good

ok

unsuitable

ok

Protein, 2D crystals, negative stained

good

good

unsuitable

ok

Protein, 2D crystals, cryo

good

good

unsuitable

unsuitable

Polymer, lighter elements

good

ok

unsuitable

unsuitable

Bacterial Suspensions

good

good

unsuitable

ok

Cell fragment suspensions

good

good

unsuitable

good

Diffraction studies

good

good

unsuitable

unsuitable

High resolution microscopy, biology

good

good

unsuitable

ok

High resolution microscopy, materials

good

good

unsuitable

ok

High temperature techniques/ heating stage

good

good

unsuitable

unsuitable

Low magnification microscopy

ok

good

ok

good

Particulate suspension, biological

ok

good

ok

good

Particulate suspension, non-biological

ok

good

ok

ok

Powders, dry

ok

good

ok

good

Replicas & low temperature techniques

good

good

ok

ok

Thin sections

ok

good

ok

good

Viral suspensions

good

good

unsuitable

unsuitable

EDS (energy dispersive spectrometry)

good

good

unsuitable

ok

 

2.1 Thin carbon film  coated grids:  

This is pure thin carbon films coated grid. The carbon film is very clean and pure. Thickness of the carbon film is less than 10nm. Since the film is very thin and fragile, it requires delicate handling during specimen preparation. We only coated it on 400 mesh grids.

Carbon: <10nm,  400mesh grids, 300 mesh grids and 200 mesh grids.

 

 

Thin carbon film coated grids, carbon film thickness is < 10nm
Prod # Description Per Grid Package Price  
Cu-400CN Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-400CN Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $6.99 $174.75, pkg/25
Cu-300CN Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-300CN Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um, the best grid for Cryo-EM. $6.99 $174.75, pkg/25
Cu-200CN Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-200CN Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

2.2 Thick carbon film  coated grids:

This is pure thick carbon films coated grid. The carbon film is very clean and pure. The thickness of the carbon film is about 35nm. Since the pure carbon film is very fragile, we don't coat them on grids less than 200 mesh

Carbon: ~35nm,  400mesh grids, 300 mesh grids, 200 mesh grids.

Thick carbon film coated grids, carbon film thickness is ~35nm
Prod # Description Per Grid Package Price  
Cu-400CK Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-400CK Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $6.99 $174.75, pkg/25
Cu-300CK Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-300CK Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $6.99 $174.75, pkg/25
Cu-200CK Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-200CK Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

 2.3  Formvar film coated grids:  

A Formvar film is covered on the grids. The thickness of the formvar film is about 20-30nm. The film is easy to be broken if it is too thin. The grid with less holes is need thicker formvar for the reason of stabilization.

Formvar: ~ 20-30nm, All  grids

 

Formvar film coated grids, film thickness is ~20-30nm on both sides.
Prod # Description Per Grid Package Price

 

Cu-400F Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $0.99 $49.50, pkg/50

Purchase Order Information

Mo-400F Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $5.99 $149.75, pkg/25
Cu-300F Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $0.99 $49.50, pkg/50

Purchase Order Information

Mo-300F Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $5.99 $149.75, pkg/25
Cu-200F Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $0.99 $49.50, pkg/50

Purchase Order Information

Mo-200F Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $5.99 $149.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

2.4  Carbon/Formvar film grids:

A Formvar film coated with a ôheavier?layer of carbon. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques. If the carbon surface is hydrophobic, specimen suspensions can be applied to the Formvar surface.

Carbon: ~15nm, Formvar: ~ 20-30nm,  All grids

 

Carbon/Formvar film coated grids, Carbon ~15nm, Formvar ~20-30nm.

Prod #

Description

Per Grid

Package Price

 

Cu-400FC Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um

$1.49

$74.50, pkg/50

Purchase Order Information

Mo-400FC Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um

$4.99

$174.75, pkg/25

Cu-300FC Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um

$1.49

$74.50, pkg/50

Purchase Order Information

Mo-300FC Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um

$6.99

$174.75, pkg/25

Cu-200FC Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um

$1.49

$74.50, pkg/50

Purchase Order Information

Mo-200FC Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um

$6.99

$174.75, pkg/25

For other kind of thin carbon coated grids, please email for quotation request.

 

 

3. Holey Film Coated Grids:

 

Although the carbon film could be very thin and usually presents no big problem in obscuring a sample, for high resolution studies of unstained biological macromolecules any interference on the beam can be problematic. For high resolution studies, holey grids are used in conjunction with freeze-sample (cryo) electron microscopy. Similar to normal carbon coated grids, holey grids are covered with a fine layer of carbon. However, as part of the preparation process, the carbon  film is deposited in such a way that there are holes of a desired size in the carbon.

 

One of the purposes of these holes is to eliminate any absorption and scattering of the electron beam by the carbon film, which will generate noise and obstruct the signal. Because cryo EM does not use staining, any elimination of background noise is desirable. The holes also allow for "pockets" of solvent to form. Within these pockets, the specimen remains fully hydrated, even when the sample has been frozen.

 

A problem can arise when holey grids are used in conjunction with proteins that are positively charged, such as DNA binding proteins. Because carbon film has a slight negative charge, especially after glow discharging, it readily attracts positively charged proteins. Therefore, such a protein will preferentially land on the carbon as opposed to the holes. A workaround has been developed using a secondary carbon layer. A holey grid is made as described above. Before depositing the sample, however, a very thin layer of carbon is deposited above the original film. Because this carbon is continuous, there is no preference to where the protein will lay down. Furthermore, the layer is thin enough to not have a great impact on the signal to noise ratio of the sample.


Following table will help you determine what holey film to use for your particular transmission electron microscopy project. Once you have decided which substrate best suits your needs, you can go on on this page for a listing of available support films and pricing.

 

 

1

2

3

4

5

Application

thin holey

carbon film

thick holey

carbon film

holey carbon

/carbon film

holey formvar

film

holey carbon

/formvar film

Protein, single particle, negative stained

unsuitable

unsuitable

unsuitable

unsuitable

unsuitable

Protein, single particle, cryo

good

good

good

ok

good

Protein, 2D crystals, negative stained

unsuitable

unsuitable

unsuitable

unsuitable

unsuitable

Protein, 2D crystals, cryo

good

good

good

unsuitable

good

Polymer, lighter elements

good

good

good

unsuitable

good

Bacterial Suspensions

good

good

good

unsuitable

ok

Cell fragment suspensions

good

good

good

unsuitable

good

Diffraction studies

good

good

good

ok

ok

High resolution microscopy, biology

good

good

good

unsuitable

good

High resolution microscopy, materials

good

good

good

unsuitable

good

High temperature techniques/ heating stage

good

good

good

unsuitable

unsuitable

Low magnification microscopy

good

good

good

ok

good

Particulate suspension, biological

good

good

good

ok

good

Particulate suspension, non-biological

good

good

good

ok

ok

Powders, dry

good

good

good

ok

good

Replicas & low temperature techniques

good

good

good

ok

good

Thin sections

good

good

good

ok

good

Viral suspensions

good

good

good

unsuitable

good

EDS (energy dispersive spectrometry)

good

good

good

unsuitable

good

 

The hole size in the holey films is from .5um to 10um. The available holey film grids are as below,

 

3.1  Thin holey carbon film coated grids:

This is pure holey carbon films coated grid. The holey carbon film is very clean and pure. The thickness of the carbon film is about 15nm. The hole size is from 0.2um to 8um. Since the pure carbon film is very fragile, we don't coated them on grids less than 200 mesh. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques

Carbon: ~15nm,  holes: 0.2um-4um (~80% small holes, ~20% large holes), 400mesh grids, 300 mesh grids, 200 mesh grids.

 

Thin holey carbon film coated grids, carbon film thickness is ~15nm, holes is from 0.2um to 8um
Prod # Description Per Grid Package Price

 

Cu-400HN Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-400HN Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $7.99 $199.75, pkg/25
Cu-300HN Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-300HN Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $7.99 $199.75, pkg/25
Cu-200HN Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-200HN Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

 

3.2  Thick holey carbon film coated grids:

This is pure holey carbon films coated grid. The holey carbon film is very clean and pure. The thickness of the carbon film is about 30nm. The hole size is from 0.2um to 8um. Since the pure carbon film is very fragile, we don't coated them on grids less than 200 mesh. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques

Carbon: ~30nm,  holes: 0.2um-4um  (~80% small holes, ~20% large holes), 400mesh grids, 300 mesh grids, 200 mesh grids.

 

Thick holey carbon film coated grids, carbon film thickness is ~30nm, holes is from 0.2um to 8um
Prod # Description Per Grid Package Price

 

Cu-400HK Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-400HK Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $7.99 $199.75, pkg/25
Cu-300HK Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-300HK Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $7.99 $199.75, pkg/25
Cu-200HK Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-200HK Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

3.3  Holey carbon film and continue carbon films (double films) coated grids:

Pure carbon support films with no Formvar backing. This new product has an even thinner carbon film which is mounted on a carbon holey film. The carbon support layer lying over the holes is less than 3nm in thickness and is the thinnest support film available. It is particularly useful for high resolution microscopy of low contrast particles and is also ideal for use with the Energy Filtering TEM.

the holey film layer is with hole size of 0.2 to 10um. Since the pure carbon film is very fragile, we don't coated them on grids less than 200 mesh.

Continue carbon films: ~5nm, holey carbon film layer: ~30nm, holes size: .2um-10um  (~80% small holes, ~20% large holes).

 

Holey/continue carbon double films coated grids, carbon: ~5nm and ~30nm, holes ~ 0.2-10um
Prod # Description Per Grid Package Price

 

Cu-400HD Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-400HD Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $9.99 $249.75, pkg/25
Cu-300HD Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-300HD Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $9.99 $249.75, pkg/25
Cu-200HD Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-200HD Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $9.99 $249.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

 

3.4  Holey Formvar film coated grids:  

A holey formvar film is covered on the grids. This is a resilient, all-purpose specimen support film, ideal for mounting thin sections and for applications using lower ranges of magnification. The thickness of the formvar film is about 50nm.

Formvar: ~ 50nm, All  grids

 

Holey formvar film coated grids, film ~50nm, hole 0.5-10um
Prod # Description Per Grid Package Price

 

Cu-400HF Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-400HF Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $7.99 $199.75, pkg/25
Cu-300HF Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $1.99 $49.75, pkg/50

Purchase Order Information

Mo-300HF Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $7.99 $199.75, pkg/25
Cu-200HF Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $1.99 $49.75, pkg/25

Purchase Order Information

Mo-200HF Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

3.5  Holey carbon/formvar film coated grids

A holey formvar film and holey carbon film are covered on the grids. The heat and electrical conducting properties of carbon help to stabilize the Formvar films when exposed to the electron beam. This is a resilient, all-purpose specimen support film, ideal for mounting thin sections and for applications using lower ranges of magnification. The thickness of the formvar film is about 50nm. The film is easy to be broken if it is too thin. The grid with less holes is need thicker formvar for the reason of stabilization.

Carbon: ~15nm, Formvar: ~ 50nm, All  grids

 

Holey carbon/formvar film coated grids, film ~50nm, hole 0.5-10um
Prod # Description Per Grid Package Price

 

Cu-400HFC Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $2.49

$62.25, pkg/25

Purchase Order Information

Mo-400HFC Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $7.99

$199.75, pkg/25

Cu-300HFC Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $2.49

$62.25, pkg/25

Purchase Order Information

Mo-300HFC Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $7.99

$199.75, pkg/25

Cu-200HFC Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.49

$62.25, pkg/25

Purchase Order Information

Mo-200HFC Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99

$199.75, pkg/25

For other kind of thin carbon coated grids, please email for quotation request.

 

 
4. Lacey Film Coated Grids:

 

A lacey network support film. The holes in the lacey support film vary in size from less than a quarter micron to more than 10 microns making them ideal for any type of specimen. Lacey support films are extremely strong and withstand vigorous specimen preparation treatment. The specimen material is supported by the film network but lies across or protrudes into the holes of the mesh. This allows high definition imaging without the effects of underlying support material.
 

Lacey films can be used for specimens ranging from large crystals and other particulate material to virus particles. Smaller particles, such as viruses or bacteria, tend to adhere around the inner edges of the holes, an ideal situation for high resolution microscopy. Lacey films are also ideal for selected area electron diffraction imaging. We offer two types of lacey film:

 

A problem can arise when holey grids are used in conjunction with proteins that are positively charged, such as DNA binding proteins. Because carbon film has a slight negative charge, especially after glow discharging, it readily attracts positively charged proteins. Therefore, such a protein will preferentially land on the carbon as opposed to the holes. A workaround has been developed using a secondary carbon layer. A holey grid is made as described above. Before depositing the sample, however, a very thin layer of carbon is deposited above the original film. Because this carbon is continuous, there is no preference to where the protein will lay down. Furthermore, the layer is thin enough to not have a great impact on the signal to noise ratio of the sample


Following table will help you determine what lacey film to use for your particular transmission electron microscopy project. Once you have decided which substrate best suits your needs, you can go on on this page for a listing of available support films and pricing.

 

 

1

2

Application lacey carbon  film lacey/thin carbon film

Protein, single particle, negative stained

unsuitable

good

Protein, single particle, cryo

good

good

Protein, 2D crystals, negative stained

unsuitable

good

Protein, 2D crystals, cryo

good

good

Polymer, lighter elements

good

good

Bacterial Suspensions

good

good

Cell fragment suspensions

ok

good

Diffraction studies

ok

good

High resolution microscopy, biology

good

good

High resolution microscopy, materials

ok

good

High temperature techniques/ heating stage

good

good

Low magnification microscopy

ok

good

Particulate suspension, biological

ok

good

Particulate suspension, non-biological

ok

good

Powders, dry

ok

good

Replicas & low temperature techniques

good

good

Thin sections

ok

good

Viral suspensions

good

good

EDS (energy dispersive spectrometry)

good

good

 

The hole size in the holey films is from .5um to 10um. The available holey film grids are as below,

 

4.1  Lacey carbon film coated grids:

This is pure lacey carbon films coated grid. The holey carbon film is very clean and pure. The thickness of the carbon film is about 30nm. The hole size is from 0.2um to 8um. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques

Carbon: ~30nm,  holes: 0.2um-4um  (~80% large holes, ~20% small holes), 400mesh grids, 300 mesh grids, 200 mesh grids.

 

Lacey carbon film coated grids, carbon film thickness is ~30nm, holes is from 0.2um to 8um
Prod # Description Per Grid Package Price

 

Cu-400LC Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-400LC Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $7.99 $199.75, pkg/25
Cu-300LC Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-300LC Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um. $7.99 $199.75, pkg/25
Cu-200LC Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $2.99 $74.75, pkg/25

Purchase Order Information

Mo-200LC Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $7.99 $199.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

4.2  Lacey/thin carbon film coated grids:

This is a double layers of films coated grid, a lacey film and thin continue carbon film. The thickness of the carbon film is about 5nm. The layer film is with hole size of 0.2 to 10um. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques.

carbon film: ~5-10nm, lacey film: ~30nm, holes size: .2um-10um (~80% large holes, ~20% small holes)

 

Lacey carbon/carbon film coated grids, film thickness: ~30nm/5nm, holes ~ 0.2-10um
Prod # Description Per Grid Package Price

 

Cu-400LD Copper grids, 400 mesh, 3.05mm O.D. hole size: ~42um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-400LD Molybdenum, 400 mesh, 3.05mm O.D. hole size: ~42um $9.99 $249.75, pkg/25
Cu-300LD Copper grids, 300 mesh, 3.05mm O.D. hole size: ~63um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-300LD Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um $9.99 $249.75, pkg/25
Cu-200LD Copper grids, 200 mesh, 3.05mm O.D. hole size: ~97um $3.99 $99.75, pkg/25

Purchase Order Information

Mo-200LD Molybdenum, 200 mesh, 3.05mm O.D. hole size: ~97um $9.99 $249.75, pkg/25
For other kind of thin carbon coated grids, please email for quotation request.

 

 

5. Quantifoil Film Coated Grids:

 

Four reasons to use Quantifoil:
1. With the known size of the pore of the Quantifoil, one can estimate the size of a particle observed directly.

2. With the defined size and pitch of the holes in the Quantifoil one can use automated electron microscopy for the first time.

3. The Quantifoil 7/2 gives a good portion of "free" area.
 

4. Hole size and hole distribution uniform over a wide range

 

These grids have been cleaned and trested very carefully, therefore, no any plastics film and less charging under electron beam


Following table will help you determine what lacey film to use for your particular transmission electron microscopy project. Once you have decided which substrate best suits your needs, you can go on on this page for a listing of available support films and pricing.

 

 

1

2

Application

Quantifoil Quantifoil/thin carbon film

Protein, single particle, negative stained

unsuitable

good

Protein, single particle, cryo

good

good

Protein, 2D crystals, negative stained

unsuitable

good

Protein, 2D crystals, cryo

good

good

Polymer, lighter elements

good

good

Bacterial Suspensions

good

good

Cell fragment suspensions

ok

good

Diffraction studies

ok

good

High resolution microscopy, biology

good

good

High resolution microscopy, materials

ok

good

High temperature techniques/ heating stage

good

good

Low magnification microscopy

ok

good

Particulate suspension, biological

ok

good

Particulate suspension, non-biological

ok

good

Powders, dry

ok

good

Replicas & low temperature techniques

good

good

Thin sections

ok

good

Viral suspensions

good

good

EDS (energy dispersive spectrometry)

good

good

 

The hole size in the holey films is from .5um to 10um. The available holey film grids are as below,

 

5.1  QUANTIFOIL?/sup> coated grid

 

QUANTIFOIL?/sup> with circular holes is mainly used in cryo-EM. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. Some information about the preparation of vitrified specimens as well as some other application examples can be found on our applications page. The hole size that is chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.

 

Our Quantifoil grids were all well treated with removing plastics film from the original products, therefore, they are much more satiable under electron beam.

 

Quantifoil R1/4 (hole:~1um; bar:~4um)

 

Quantifoil R1.2/1.3 (hole:~1.2um; bar:~1.3um)

Cu-400QC1/4, Cu grid, 400 mesh,

$199.75, pkg/25,

 

Cu-400QC1/2, Cu grid, 400 mesh,

$199.75, pkg/25,

Cu-300QC1/4, Cu grid, 300 mesh,

$199.75, pkg/25,

 

Cu-300QC1/2, Cu grid, 300 mesh,

$199.75, pkg/25,

Cu-200QC1/4, Cu grid, 200 mesh,

$199.75, pkg/25,

 

Cu-200QC1/2, Cu grid, 200 mesh,

$199.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil R2/1 (hole:~2um; bar:~1um)

 

Quantifoil R2/2 (hole:~1um; bar:~1um)

Click to enlarge

Cu-400QC2/1, Cu grid, 400 mesh,

$199.75, pkg/25,

 

Cu-400QC2/2, Cu grid, 400 mesh,

$199.75, pkg/25,

Cu-300QC2/1, Cu grid, 300 mesh,

$199.75, pkg/25,

 

Cu-300QC2/2, Cu grid, 300 mesh,

$199.75, pkg/25,

Cu-200QC2/1, Cu grid, 200 mesh,

$199.75, pkg/25,

 

Cu-200QC2/2, Cu grid, 200 mesh,

$199.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil R2/4 (hole:~2um; bar:~4um)

 

Quantifoil R3.5/1 (hole:~3.5um; bar:~`um)

Cu-400QC2/4, Cu grid, 400 mesh,

$199.75, pkg/25,

 

Cu-400QC3/1, Cu grid, 400 mesh,

$199.75, pkg/25,

Cu-300QC2/4, Cu grid, 300 mesh,

$199.75, pkg/25,

 

Cu-300QC3/1, Cu grid, 300 mesh,

$199.75, pkg/25,

Cu-200QC2/4, Cu grid, 200 mesh,

$199.75, pkg/25,

 

Cu-200QC3/1, Cu grid, 200 mesh,

$199.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil S7/2 (hole:~7um; bar:~1um)

 

Quantifoil Multi A (various hole size)

Cu-400QC7/2, Cu grid, 400 mesh,

$199.75, pkg/25,

 

Cu-400QCM, Cu grid, 400 mesh,

$199.75, pkg/25,

Cu-300QC7/2, Cu grid, 300 mesh,

$199.75, pkg/25,

 

Cu-300QC, Cu grid, 300 mesh,

$199.75, pkg/25,

Cu-200QC7/2, Cu grid, 200 mesh,

$199.75, pkg/25,

 

Cu-200QCM, Cu grid, 200 mesh,

$199.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

5.2  Quantifoil/thin carbon film coated grids:

 

Quantifoil R1/4 with thin carbon film

 

Quantifoil R1.2/1.3 with thin carbon film

Cu-400QC1/4N, Cu grid, 400 mesh,

$249.75, pkg/25,

 

Cu-400QC1/2N, Cu grid, 400 mesh,

$249.75, pkg/25,

Cu-300QC1/4N, Cu grid, 300 mesh,

$249.75, pkg/25,

 

Cu-300QC1/2N, Cu grid, 300 mesh,

$249.75, pkg/25,

Cu-200QC1/4N, Cu grid, 200 mesh,

$249.75, pkg/25,

 

Cu-200QC1/2N, Cu grid, 200 mesh,

$249.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil R2/1 with thin carbon film

 

Quantifoil R2/2 with thin carbon film

Click to enlarge

Cu-400QC2/1N, Cu grid, 400 mesh,

$249.75, pkg/25,

 

Cu-400QC2/2N, Cu grid, 400 mesh,

$249.75, pkg/25,

Cu-300QC2/1N, Cu grid, 300 mesh,

$249.75, pkg/25,

 

Cu-300QC2/2N, Cu grid, 300 mesh,

$249.75, pkg/25,

Cu-200QC2/1N, Cu grid, 200 mesh,

$249.75, pkg/25,

 

Cu-200QC2/2N, Cu grid, 200 mesh,

$249.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil R2/4 with thin carbon film

 

Quantifoil R3.5/1 with thin carbon film

Cu-400QC2/4N, Cu grid, 400 mesh,

$249.75, pkg/25,

 

Cu-400QC3/1N, Cu grid, 400 mesh,

$249.75, pkg/25,

Cu-300QC2/4N, Cu grid, 300 mesh,

$249.75, pkg/25,

 

Cu-300QC3/1N, Cu grid, 300 mesh,

$249.75, pkg/25,

Cu-200QC2/4N, Cu grid, 200 mesh,

$249.75, pkg/25,

 

Cu-200QC3/1N, Cu grid, 200 mesh,

$249.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 

Quantifoil S7/2 with thin carbon film

 

Quantifoil Multi A with thin carbon film

Cu-400QC7/2N, Cu grid, 400 mesh,

$249.75, pkg/25,

 

Cu-400QCMN, Cu grid, 400 mesh,

$249.75, pkg/25,

Cu-300QC7/2N, Cu grid, 300 mesh,

$249.75, pkg/25,

 

Cu-300QCMN, Cu grid, 300 mesh,

$249.75, pkg/25,

Cu-200QC7/2N, Cu grid, 200 mesh,

$249.75, pkg/25,

 

Cu-200QCMN, Cu grid, 200 mesh,

$249.75, pkg/25,

We also have the Nickel, Gold, Cu/Rh grids coated with these kind of films, email us for quotation.

 
6. Grid Storage Box:

 

Ten Features for you to use it:

1. Cross-shape holes: Support for left/right hands.
2. Wheel shape female metal screw: release thermal extension power, never break.
3. Box size: fit to FEI vitrobot, vary cryo-EM transfer stages, easy to tighten/release the cap.
4. Screw cap: Fit to both Slot/Philips drivers.
5. Hole shape: Easy to pickup and release grids.

6. Overall box shape: easy to name and number
7. High transparency cap: easy to check the visualize the grid inside hole.
8. Male metal screw: never worry to break it by over tighten.
9. White box color: easy to mark and with a good visibility in liquid N2.
10. Metal male and female screw: Anti-freeze under liquid N2 temperature.

 

TEM Grid Boxes

Prod # Description Per box Package Price
GB-4R 4-holes Cryo-EM storage box (round shape). $5.95 $5.95, pkg /1 box

 Purchase Order Information
GB-4S 4-hole Cryo-EM storage box (square shape). $4.95 $4.95, pkg /1 box

 Purchase Order Information
GB-100 100-hole TEM grid storage box $6.95 $6.95, pkg /1 box

 Purchase Order Information
GB-50 50-hole TEM grid storage box $5.95 $5.95, pkg /1 box

 Purchase Order Information

 

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