Pacific Grid Tech, a high quality TEM grid provider, TEM grid, EM grid, eletron microscope grid, Mo grid, Molybdenum grid, Copper grid, Cu grid, Nicle grid, Ni grid, Au grid, grid, holey grid, holey film grid, carbon film grid, grid coated with Carbon film, flat film grid, holey film, Lacey grid, lacey film grid, Quantifoil grid, Quantifoil, pacific grid tech, Pacific Grid-Tech, Pacific GridTech, grid-tech, gridtech, grid tech, smooth surface grid, grid, 2-D crystals, smooth surface, cryo-EM grid, cryo-EM, electron cryo-microscopy, 200 mesh grid, 300 mesh grid, 400 mesh, grid for 2-D crystals, grid storage box

Welcome to Pacific Grid-Tech, a high quality TEM grid provider. We produce several kinds of TEM grids, such as Mo grids, Cu grids, Ni grids... and also produce the grids pre-coated with many kinds of films, such as holey film, thin carbon film and sandwich film.

Our grids surface is very smooth, which results from the film being coated flatly. The flat film is critical to several scientific research projects, especially for electron crystallography.

What causes your high resolution diffraction spots lost? (Diffraction of Bacteriorhodopsin crystals at 45° tilt, pictures were collected on CCD camera of CM120)

Flat crystals	Almost flat crystals	Wrinkled crystals	Badly wrinkled crystals
From: Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

What could cause your crystals wrinkled?

"YE Measurements on grids made of titanium, molybdenum and tungsten showed significantly less puckering..., ... that carbon showed less cryo-crinkling on molybdenum than on titanium grids."
-- Booy F.P. etc., Cryo-crinkling: what happens to carbon films on copper grids at low temperature, Ultramicroscopy. 48(1993)3, 273-80

"... the use of molybdenum grids has become standard in electron cryo-microscopy. However, the rough surface of these grids can also induce the carbon film to crinkle..."
-- Fujiyoshi Y., The structural study of membrane proteins by electron crystallography, Adv. Biophysics, 35(1998) 25-80.

"One of the main problems in data collection of 2D protein crystals for EM is a lack of flatness of the specimens. The resulting overlap of data from crystal areas of slightly different tilt angles (visible as blurring of the diffraction spots in the direction perpendicular to the tilt axis) is especially severe at high tilt angles and effectively reduces the vertical resolution of a 3-D map"
-- Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

The surface of the carbon film on grids from different companies (SEM images of grids with Carbon-coated film)


300 mesh titanium grid (from another company)	300 mesh molybdenum grid (from another company)	300 mesh molybdenum grid (from Pacific GridTech)
From: Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

For the quality of our grids, please visit our Testimonials and Clients

Pacific Grid Tech
1 Sansome Street, 35 Floor, PMB #675
San Francisco, CA 94104
USA
Tel: (800) 945 GRID (4743)
(415) 336 5675 (International)
Fax:(415) 354 8382
Web: http://www.grid-tech.com/
EMail: info@grid-tech.com