Welcome to Pacific Grid-Tech, a high quality TEM grid provider. We product several kinds of TEM grids, such as Mo grids, Cu grids, Ni grids... and also product the grids pre-coated with many kind of films, such as holey film, thin carbon film and sandwich film. 

 

[Product Catalog]

[TEM Grid] [Continue Film Coated Grid] [Holey Film Coated Grid] [Grid Storage Box]

[Purchase Order Information]

 

Our grids surface is very smooth, which result the film coated flatly. The flat film is critical to several scientific research projects, especially for electron crystallography, In this website, you could find our products, clients' testimonials, related literatures, pricing and  purchase order information.

 

What causes your high resolution diffraction spots lost?

(Diffraction of Bacteriorhodopsin crystals at 45?tilt, pictures were collected on CCD camera of CM120)

Flat crystals

Almost flat crystals

Wrinkled crystals

Badly wrinkled crystals

 

From: Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.


What could cause your crystals wrinkled?

 

“… Measurements on grids made of titanium, molybdenum and tungsten showed significantly less puckering..., ... that carbon showed less "cryo-crinkling" on molybdenum than on titanium grids."

-- Booy F.P. etc., Cryo-crinkling: what happens to carbon films on copper grids at low temperature. Ultramicroscopy. 48(1993)3,  273-80

 

?.. the use of molybdenum grids has become standard in electron cryo-microscopy. However, ?the rough surface of these grids can also induce the carbon film to crinkle…” 

-- Fujiyoshi Y., The structural study of membrane proteins by electron crystallography, Adv. Biophysics, 35(1998) 25-80.

 

“One of the main problems in data collection of 2D protein crystals for EM is a lack of flatness of the specimens. The resulting overlap of data from crystal areas of slightly different tilt angles (visible as blurring of the diffraction spots in the direction perpendicular to the tilt axis) is especially severe at high tilt angles and effectively reduces the vertical resolution of a 3-D map.?#060;/span>

-- Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

 

How about the flatness of carbon film on grids from different companies?

(SEM images of grids with Carbon-coated film)

300 mesh titanium grid

(from other company)

300 mesh molybdenum grid

(from other company)

300 mesh molybdenum grid

(from Pacific GridTech)

 

From: Vonck, J., Parameters affecting specimen flatness of two-dimensional crystals for electron crystallography, Ultramicroscopy, 5(2000) 123-129.

 

How about our grids?

 

Please visit our webpage of Testimonials and Clients to get more information.

 

 

Pacific Grid Tech

 

1 Sansome Street, 35 Floor, PMB #675

San Francisco, CA 94104

USA

   : (800) 945 GRID (4743)

       : (415) 336 5675 (International)

  : (415) 354 8382

Web: http://www.grid-tech.com/

EMail: info@grid-tech.com

 

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