Welcome to
Pacific Grid-Tech, a high quality TEM grid
provider. We product several kinds of TEM grids, such as Mo grids, Cu grids, Ni
grids... and also product the grids pre-coated with many kind of films, such as
holey film, thin carbon film and sandwich film.
[Product Catalog]
[TEM Grid] [Continue
Film Coated Grid] [Holey Film Coated Grid]
[Grid Storage Box]
[Purchase Order Information]
Our grids surface is very smooth, which result the film coated flatly.
The flat film is critical to several scientific research projects, especially for
electron crystallography, In this website, you could find our products,
clients' testimonials, related literatures,
pricing and purchase order information.
What causes your high resolution
diffraction spots lost?
(Diffraction of Bacteriorhodopsin crystals at 45?tilt, pictures were collected on CCD camera of CM120) |
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Flat
crystals |
Almost flat crystals |
Wrinkled crystals |
Badly
wrinkled crystals |
From: Vonck, J., Parameters affecting specimen flatness of two-dimensional
crystals for electron crystallography,
Ultramicroscopy, 5(2000)
123-129. |
What could cause your crystals
wrinkled?
“… Measurements on grids made of
titanium, molybdenum and tungsten showed significantly less puckering..., ...
that carbon showed less "cryo-crinkling" on molybdenum than on titanium grids."
-- Booy F.P. etc., Cryo-crinkling: what happens to carbon films on copper
grids at low temperature.
Ultramicroscopy. 48(1993)3, 273-80
?.. the use of molybdenum
grids has become standard in electron cryo-microscopy. However, ?the rough
surface of these grids can also induce the carbon film to crinkle…”
-- Fujiyoshi Y., The structural study of membrane proteins by electron
crystallography, Adv. Biophysics, 35(1998) 25-80.
“One of the main problems in
data collection of 2D protein crystals for EM is a lack of flatness of the
specimens. The resulting overlap of data from crystal areas of slightly
different tilt angles (visible as blurring of the diffraction spots in the
direction perpendicular to the tilt axis) is especially severe at high tilt
angles and effectively reduces the vertical resolution of a 3-D map.?#060;/span>
-- Vonck, J., Parameters affecting specimen flatness of two-dimensional
crystals for electron crystallography,
Ultramicroscopy, 5(2000)
123-129.
How about the flatness of carbon
film on
grids from different companies?
(SEM images of
grids with Carbon-coated film) |
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300
mesh titanium grid
(from other company) |
300
mesh molybdenum grid
(from other company) |
300
mesh molybdenum grid
(from
Pacific GridTech)
|
From: Vonck, J., Parameters affecting specimen flatness of two-dimensional
crystals for electron crystallography,
Ultramicroscopy, 5(2000)
123-129. |
How about our
grids?
Please visit our webpage of Testimonials
and Clients to get more
information.
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