Products --- Grids for Transmission Electron Microscopy

[Plain Grids] [Carbon Film Grids] [Holey Film Grids]

[Lacey  Grids]  [Quantifoil Grids] [Catalog] [Order Info]

  Our honor product:

300 Mesh Molybdenum Grids

(An ideal grid for electron cryo-crystallography)

Prod # Description Per Grid Package Price

 

Mo-300 Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um, idel cryo-EM grid.

The grid is with very smooth surface and edges of holes, which could provide a flatness carbon film, and is an ideal grid for cryo-crystallography on membrane 2D crystals. (click here for details)

$199.99, pkg/50

Purchase Order Information

Mo-300CN Molybdenum, 300 mesh, 3.05mm O.D. hole size: ~63um, idel cryo-EM grid. Molybdenum grids pre-coated with thin and flat carbon film, 300 mesh. $6.99 $174.75, pkg/25

 

1. Plain Grids:

 

The diameter of grids is standard, as 3.05mm. The Thickness is variable based on the grid materials. The thickness range is from 10um to 25um. The mesh of the grid is defined by the number of hole within 1 inch. For example, the 200 mesh grid has 20 holes along diameter direction,  400 mesh grid has 40 holes in diameter.

 

Since the diameter of grid is a standard length, 3.05mm,  each hole size is less than ~200um in 100 mesh grid,  ~97um in 200 mesh grid, ~63um in 300 mesh grid and ~42um in 400 mesh grid.

 

Please be noticed that our grids have very smooth hole edge. The smooth edge grids could provide a very flat film coated on them, which is critical for high resolution imaging.

 

600 mesh 500 mesh 400 mesh 300 mesh 200 mesh 100 mesh 50 mesh Single hole

Copper

(Cu-600)

Cu/Rh

(Cu/Rh-600)

Nickel

 (Ni-600)

Copper

(Cu-500)

Nickel

 (Ni-500)

Copper

(Cu-400)

Cu/Rh

(Cu/Rh-400)

Molybdenum

 (Mo-400)

Nickel

 (Ni-400)

Titanium

(Ti-400)

Copper

(Cu-300)

Cu/Rh

(Cu/Rh-300)

Molybdenum

 (Mo-300)

Nickel

 (Ni-300)

Titanium

(Ti-300)

Steel

(Fe-300)

Copper (Cu-200)

Cu/Rh (Cu/Rh-200)

Molybdenum (Mo-200)

Nickel (Ni-200)

Titanium

(Ti-200)

Gold (Au-200)

Tungsten (W-200)

Steel (Fe-200)

Nylon (Ny-200)

Copper (Cu-100)

Cu/Rh (Cu/Rh-100)

Molybdenum (Mo-100)

Nickel (Ni-100)

Titanium

(Ti-100)

Gold (Au-100)

Steel (Fe-100)

Copper (Cu-50)

Molybdenum (Mo-50)

Nickel (Ni-50)

Titanium

(Ti-50)

Gold (Au-50)

Steel (Fe-50)

Copper

(Cu-s)

Molybdenum

(Mo-s)

 

2. Continue Film Coated Grids:

 

Most samples for transmission electron microscopy must be "supported" on some kind of a thin electron transparent film, to hold the specimen in place while in the objective lens of the TEM. Only samples that are "self- supporting" do not need some additional support film. Should an electron microscope user use a support film at all if one is not absolutely needed? Probably not, because even the very best quality of support films do result in more material being placed in the beam's path, resulting even if ever so slightly, the contrast of the final image. But for many users, they don't have the luxury of having to make that decision: More often than not, even what appear to be self-supporting samples, still do need the assistance of a support film to cut down on sample drift.

Mo-300CN, Molybdenum grid coated with thin carbon film.

 

The selection of the "right" support film has never been an easy exercise, even the most experienced of TEM experts, and the only way to really determine which support film is the best for a particular application it to just "try it!" Therefore, we would like to contribute in a useful way to the needed information base to help make the optimum selections, but the giving of such "guidance" itself is an imprecise science.

We have therefore broken down the different kinds of support films and have tried to present the "trade offs" between one support film system vs. another. We can offer no guarantees, since the range of samples studied is so wide and the environmental range so broad (from cryo temperatures to temperatures exceeding 1000C) to which the grids and their coatings might be subjected. But the best decisions are usually made by just trying several possibilities and if one works better than the other, the choice for future samples becomes obvious.

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Items

Diagram

Grids Availability

1. Thin carbon film  grids:

Carbon film: <15nm

Copper 400 mesh (Cu-400CN)

Copper 300 mesh (Cu-300CN)

Copper 200 mesh (Cu-200CN)

Molybdenum 400 mesh (Mo-400CN)

Molybdenum 300 mesh (Mo-300CN)

Molybdenum 200 mesh (Mo-200CN)

2. Thick carbon film  grids:

Carbon film: ~35nm,

Copper 400 mesh (Cu-400CK)

Copper 300 mesh (Cu-300CK)

Copper 200 mesh (Cu-200CK)

Molybdenum 400 mesh  (Mo-400CK)

Molybdenum 300 mesh  (Mo-300CK)

Molybdenum 200 mesh  (Mo-200CK)

3. Formvar film grids:

Formvar film: ~20-30nm

Copper 400 mesh (Cu-400F)

Copper 300 mesh (Cu-300F)

Copper 200 mesh (Cu-200F)

Copper 100 mesh (Cu-100F)

Molybdenum 400 mesh  (Mo-400F)

Molybdenum 300 mesh  (Mo-300F)

Molybdenum 200 mesh  (Mo-200F)

Molybdenum 100 mesh  (Mo-100F)

4. Carbon/Formvar film grids:

Carbon film: ~15nm;

Formvar film: ~20-30nm

Copper 400 mesh (Cu-400FC)

Copper 300 mesh (Cu-300FC)

Copper 200 mesh (Cu-200FC)

Copper 100 mesh (Cu-100FC)

Molybdenum 400 mesh  (Mo-400FC)

Molybdenum 300 mesh  (Mo-300FC)

Molybdenum 200 mesh  (Mo-200FC)

Molybdenum 100 mesh  (Mo-100FC)

 

 

3. Holey Film Coated Grids:

 

   Although the carbon film is very thin and usually presents no big problem in obscuring a sample, for high resolution studies of unstained biological macromolecules any interference on the beam can be problematic. For high resolution studies, holey grids are used in conjunction with freeze-sample (cryo) electron microscopy. Similar to normal carbon coated grids, holey grids are covered with a fine layer of carbon. However, as part of the preparation process, the carbon film is deposited in such a way that there are holes of a desired size in the carbon.

   One of the purposes of these holes is to eliminate any absorption and scattering of the electron beam by the carbon film, which will generate noise and obstruct the signal. Because cryo EM does not use staining, any elimination of background noise is desirable. The holes also allow for "pockets" of solvent to form. Within these pockets, the specimen remains fully hydrated, even when the sample has been frozen.

   A problem can arise when holey grids are used in conjunction with proteins that are positively charged, such as DNA binding proteins. Because carbon film has a slight negative charge, especially after glow discharging, it readily attracts positively charged proteins. Therefore, such a protein will preferentially land on the carbon as opposed to the holes. A workaround has been developed using a secondary carbon layer. A holey grid is made as described above. Before depositing the sample, however, a very thin layer of carbon is deposited above the original film. Because this carbon is continuous, there is no preference to where the protein will lay down. Furthermore, the layer is thin enough to not have a great impact on the signal to noise ratio of the sample.

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Items

Diagram

Grids Availability

1. Thick carbon holey grids:

Carbon: ~35nm

Copper 400 mesh (Cu-400HK)

Copper 300 mesh (Cu-300HK)

Copper 200 mesh (Cu-200HK)

Molybdenum 400 mesh  (Mo-400HK)

Molybdenum 300 mesh  (Mo-300HK)

Molybdenum 200 mesh  (Mo-200HK)

2. Holy/thin films  grids:

Carbon 1: ~15nm

Carbon 2: ~15nm

Copper 400 mesh (Cu-400HD)

Copper 300 mesh (Cu-300HD)

Molybdenum 400 mesh  (Mo-400HD)

Molybdenum 300 mesh  (Mo-300HD)

3. Formvar holey grids:

Formvar: ~100nm

Copper 400 mesh (Cu-400HF)

Copper 300 mesh (Cu-300HF)

Copper 200 mesh (Cu-200HF)

Copper 100 mesh (Cu-100HF)

Molybdenum 400 mesh  (Mo-400HF)

Molybdenum 300 mesh  (Mo-300HF)

Molybdenum 200 mesh  (Mo-200HF)

Molybdenum 100 mesh  (Mo-100HF)

4. Carbon/Formvar holey grids:

Carbon: ~15nm

Formvar: ~100nm

Copper 400 mesh (Cu-400FC)

Copper 300 mesh (Cu-300FC)

Copper 200 mesh (Cu-200FC)

Copper 100 mesh (Cu-100FC)

Molybdenum 400 mesh  (Mo-400FC)

Molybdenum 300 mesh  (Mo-300FC)

Molybdenum 200 mesh  (Mo-200FC)

Molybdenum 100 mesh  (Mo-100FC)

 

4. Lacey Film Coated Grids:

 

   A lacey network support film. The holes in the lacey support film vary in size from less than a quarter micron to more than 10 microns making them ideal for any type of specimen. Lacey support films are extremely strong and withstand vigorous specimen preparation treatment. The specimen material is supported by the film network but lies across or protrudes into the holes of the mesh. This allows high definition imaging without the effects of underlying support material.

   Lacey films can be used for specimens ranging from large crystals and other particulate material to virus particles. Smaller particles, such as viruses or bacteria, tend to adhere around the inner edges of the holes, an ideal situation for high resolution microscopy. Lacey films are also ideal for selected area electron diffraction imaging. We offer three types of lacey film:

  A problem can arise when holey grids are used in conjunction with proteins that are positively charged, such as DNA binding proteins. Because carbon film has a slight negative charge, especially after glow discharging, it readily attracts positively charged proteins. Therefore, such a protein will preferentially land on the carbon as opposed to the holes. A workaround has been developed using a secondary carbon layer. A holey grid is made as described above. Before depositing the sample, however, a very thin layer of carbon is deposited above the original film. Because this carbon is continuous, there is no preference to where the protein will lay down. Furthermore, the layer is thin enough to not have a great impact on the signal to noise ratio of the sample.

Click here to view all product.

 

Items

Diagram

Grids Availability

2. Lacey  grids:

Carbon: ~35nm

Copper 400 mesh (Cu-400LK)

Copper 300 mesh (Cu-300LK)

Copper 200 mesh (Cu-200LK)

Molybdenum 400 mesh  (Mo-400LK)

Molybdenum 300 mesh  (Mo-300LK)

Molybdenum 200 mesh  (Mo-200LK)

3. Lacey/thin films  grids:

Carbon 1: ~15nm

Carbon 2: ~35nm

Copper 400 mesh (Cu-400LD)

Copper 300 mesh (Cu-300LD)

Copper 300 mesh (Cu-300LD)

Molybdenum 400 mesh  (Mo-400LD)

Molybdenum 300 mesh  (Mo-300LD)

Molybdenum 300 mesh  (Mo-300LD)

 

5. Quantifoil Film Coated Grids:

 

  Four reasons to use Quantifoil:
1. With the known size of the pore of the Quantifoil, one can estimate the size of a particle observed directly.
2. With the defined size and pitch of the holes in the Quantifoil one can use automated electron microscopy for the first time.
3. The Quantifoil 7/2 gives a good portion of "free" area.
4. Hole size and hole distribution uniform over a wide range

  These grids have been cleaned and trested very carefully, therefore, no any plastics film and less charging under electron beam.

Click here to view all product.

 

Items

Diagram

Grids Availability

1. Quantifoil grids:

Carbon: ~35nm

Copper 400 mesh (Cu-400QC)

Copper 300 mesh (Cu-300QC)

Copper 200 mesh (Cu-200QC)

Molybdenum 400 mesh  (Mo-400QC)

Molybdenum 300 mesh  (Mo-300QC)

Molybdenum 200 mesh  (Mo-200QC)

2. Quantifoil/thin carbon films  grids:

Carbon 1: ~35nm

Carbon 2: ~5nm

Copper 400 mesh (Cu-400QD)

Copper 300 mesh (Cu-300QD)

Copper 300 mesh (Cu-200QD)

Molybdenum 400 mesh  (Mo-400QD)

Molybdenum 300 mesh  (Mo-300QD)

Molybdenum 300 mesh  (Mo-200QD)

 

 

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